Anther Culture Pdf

PDF) Anther culture of Solanum genotypes

Notes on Anther Culture

Kyo M, Harada H Control of the developmental pathway of tobacco pollen in vitro. Springer, Berlin Google Scholar.

Caglar G, Abak K In vitro application of haploid cucumber plants. Kopecky D, Vagera J The use of mutagens to increase the efficiency of the androgenic progeny production in Solanum nigrum. It is also apparent that liquid medium is superior to solid medium for embryoid production in the responsive genotypes examined.

Raghavan V Molecular embryology of flowering plants. Both generative and vegetative cell divide further to the development of sporophyte. SlideShare Explore Search You. Murashige T, Skoog F A revised medium for rapid growth and bioassays with tobacco tissue cultures. Iron is particularly critical.

Plant Tissue

Are you sure you want to Yes No. The beneficial effects of charcoal on the response of and was demonstrated.

Broughton S Ovary co-culture improves embryo and green plant production in anther culture of Australian spring wheat Triticum aestivum L. Thus any recessive mutation or characteristic are apparent. Now customize the name of a clipboard to store your clips. General observations and effects of physical factors. Powell W Environmental and genetic aspects of pollen embryogenesis.

Activated charcoal has been used in anther cultures of dihaploid S. Tsay H-S Effects of nitrogen supply to donor plants on pollen embryogenesis in cultured tobacco anthers. Anthers were examined after six weeks in culture and scored for embryoid production. This feature of the data focuses attention on a relatively neglected area of tissue culture experimentation i. Genotype by media interaction has been reported in a number of tissue culture experiments, blank political map of india pdf although this is the first quantitative evaluation of their importance in potato cell culture experi- ments.

In some cases, extracts of the anther wall have been added to the medium or conditioned medium e. International Rice Research Newsletter Vol.

This method is specific for given species such as barley. Similarly, a large component of the genotype x pre-treatment items may be explained by the differential response of the tuberosum clones and to high and low temperature pre-treatment. Chen Z A study on induction of plants from Citrus pollen. However, much more complex methods have had to be developed to culture isolated microspores. Powell W Environmental and genetical aspects of pollen embryogenesis.


Clones were either grown from tubers or true seeds S. The tuberosum genotypes used in this study were not a random sample but a selected sub-set of genotypes known to be responsive in culture. Sunderland N Anther culture as means of haploid induction.

One can produce homozygous diploid or polyploid plants that may be valuable in plant breeding. The second pollen mitosis may occur after pollen germinates on a stigma. Verify that the link points to the correct file and location. The file may have been moved, renamed, or deleted.

Various temperature stress pre-treatment of excised floral parts have been devised to improve the anther culture response of many species. Capsicum Newsl Google Scholar. Mineral salts are needed for growth past the globular stage of embryo development. An induced haploid mutant. The relationship between sucrose concentration and embryoid produc- tion after transformation for the three genotypes is presented in graphical form in Fig.

PDF) Anther culture of Solanum genotypes

Notes on Anther Culture

Chambonnet D Production of haploid pepper plants. Clipping is a handy way to collect important slides you want to go back to later. Chromosome elimination following interspecific hybridization. Phenotypic characterization and nuclear microsatellite analysis reveal genomic changes and rearrangements underlying androgenesis in tetraploid potatoes Solanum tuberosum L. Both and display negative linear regressions i.

Anther and Pollen Culture Meaning and Principle

Mean embryoid production for the three genotypes cultured in the five concentrations of sucrose at the three different pre-treatment regimes. They carry only one allele of each gene. Prior to anther culture, excised flower buds were subjected to one of three pre-treatments.

These parameters have been scaled to sum to and were obtained from the expected mean squares appropriate to the experiment. You can change your ad preferences anytime. Kluwer, Dordrecht Google Scholar. Pre-treatment effects on embryoid induction were also inves- tigated and two of the genotypes displayed differential response to temperature stress pre- treatment. Bohanec B Ploidy determination using flow cytometry.

It is therefore reasonable to conclude that S. Heberle-Bors E, Reinert J Environmental control and evidence for predetermination of pollen embryogenesis in Nicotiana tabacum pollen. This division forms two asymmetric cells - generative and vegetative.

Sunderland N Strategies in the improvement of yields in anther culture. Show related SlideShares at end. Visibility Others can see my Clipboard. For each clone, a total of anthers were cultured per replicate. There was a positive relationship between sucrose concentration and embryoid production for S.

Apologies are extended to those authors who have not been mentioned. The uninucleate microspore undergoes a normal division but pollen embryos are formed from generative cell alone. Nowadays, these biotechnologies represent an integral part of the breeding programmes of many agronomically important crops. Tissue or callus comprises a chimera of diploid, tetraploid and haploid cells. Acta Physiologiae Plantarum.